Does Pre Workout Boost Testosterone? What Does Science Say?

It can be frustrating for men to deal with an issue related to low T or testosterone. This condition has been associated with many health concerns, including changes in mood, weight gain, low sex drive, and more.

While testosterone levels decline with age, relying on outside influences may boost your T levels. One of the factors may be a pre-workout supplement.

We’ve done the research to give you everything you need to know about pre-workouts and whether they can boost T levels. But first, let’s find out the causes of low testosterone.

What Causes Low Testosterone?

Low-T, or male hypogonadism, is a condition in which testicles don’t produce enough testosterone. The primary cause of this condition is age because testosterone production slows as a man ages. 

Other conditions that may cause low-T in males are:

  • Alcohol and drug abuse
  • Testicular infection or injury
  • Radiation cancer treatments or chemotherapy
  • Medications, such as opiate pain relief and corticosteroids
  • Genetic conditions (i.e., Klinefelter syndrome)
  • Hormone disorders or pituitary gland disease

Testicular disorder and pituitary or hypothalamus dysfunction are other causes of low testosterone.

Meanwhile, in females, low T may result from surgical removal of the ovaries, adrenal gland insufficiency, and taking oral estrogen therapy.

Low testosterone’s common downsides are difficulty building lean muscle mass, loss of energy, low self-esteem, and erectile dysfunction.

So, Does Pre Workout Boost Testosterone Levels?

Does pre-workout boost testosterone?

(Source: Pexels)

Not all, but there are pre-workout supplements that boost testosterone levels. These supplements often contain the same ingredients found in testosterone boosters. These ingredients include ashwagandha, d-aspartic acid, and caffeine.

How do they affect your T levels? Let’s find out what science says.

Ingredients that have a positive influence on testosterone

Ashwagandha is a non-toxic plant that has been a significant part of the centuries-old system of medicine called Ayurveda. It’s also been used to treat various conditions, like insomnia and rheumatism. [1]

Based on a study involving men aged 40 to 70, it has also increased testosterone by about 15% on average. [2] 

Another study suggests that ashwagandha root may help with forms of men infertility. This ingredient has been used as an anti-stress agent because it reduces cortisol levels and stress response. [3] 

Meanwhile, d-aspartic acid (DAA) is an amino acid in testosterone production. Some studies revealed that it increased testosterone levels by about 42%, while others claimed it has not. [4-5] 

Science likewise shows that when DAA is administered into the system, it activates the testes, pituitary gland, and hypothalamus. As a result, this builds up luteinizing hormone (LH), growth hormone (GH), and follicle-stimulating hormone (FSH). No wonder DAA is common in many T-booster supplements.

Then, there’s caffeine in pre-workouts and testosterone boosters. Science says men who regularly consume caffeine are more likely to have higher testosterone hormones and lower free estradiol, a form of estrogen. [6]

In a separate randomized trial published in the International Journal of Sports Nutrition and Exercise Metabolism, authors found that caffeine can positively affect testosterone levels by about 21%. However, the opposite effect in cortisol may counteract this effect. [7] 

A disruption in testosterone and cortisol balances can be linked to a sex hormone-binding globulin imbalance, which further affects the metabolic processes.

Furthermore, a randomized trial on the effects of decaffeinated and caffeinated coffee on Endogenous Sex Hormone Levels and Sex Hormone-Binding Globulin (SHBG) found that men experienced an increase in their T levels after four weeks of consuming caffeinated coffee. [8] 

Then, other ingredients prepare you for an intense workout and increase your free testosterone levels. These are:

  • L-Carnitine – protects the muscle and causes a natural drop of testosterone in the body during hard training. [9] 
  • Citrulline malate – is an amino acid with blood vessel dilating properties, which helps men overcome erectile dysfunction. It has also been shown to improve sex drive and erection strength. [10] 
  • Vitamin D and Zinc – are both critical for healthy testosterone production. [11-12]
  • Ginseng – research on prostate health has tightly associated ginseng with testosterone levels [13] 

Therefore, it’s essential to check the label of your supplements. Doing this determines whether they will help reduce or increase testosterone in the human body.

Pre-workouts get you pumped to train and not prioritize testosterone boosting. They also help improve muscle growth and performance, increase energy levels, reduce fatigue, improve mental clarity and focus, and improve muscle blood flow. 

These supplements differ completely from sports enhancers, like testosterone boosters and fat burners. However, some pre-workouts attack fats. Check out my review of the best pre-workout fat burner to learn more.

What to Avoid With Pre-Workouts

Remember that there are a few things you can’t mix with pre-workouts, like medications. These include:

  • Stimulants and caffeinated drinks
  • Milk
  • Heavy metals
  • Alcohol
  • Fatty smoothies

Effects of Pre-Workouts on Testosterone

There are two ways pre-workouts affect testosterone hormones in the body. 

Decreased Conversion

First, some ingredients in the pre-workout inhibit the effect of aromatase, which converts testosterone into estrogen. 

Increased Production

Another way pre-workout impacts testosterone in the body is it increases its production. Natural herbs, amino acids, vitamins, and minerals help the natural testosterone process. 

Some ingredients, like zinc, also ensure enough building blocks to produce fresh testosterone.

Through a T-booster or pre-workout, both processes can positively affect sex drive and erectile dysfunction and build muscle tissue.

3 Best Pre-Workout Supplements to Increase Testosterone Levels

1. C4 Sport Original

(Source: Amazon)

First on our list of the best pre-workout supplements that increase testosterone levels is the C4 Sport by Cellucor. C4 Original was launched in 2011 and is now known to be the number one pre-workout in America as it offers excellent value for its price.

This product fuels energy, focus, and endurance with 150mg of caffeine. With beta-alanine content can also power you through training, support muscular endurance, and combat muscular fatigue.

Just a caution, though, a few men experienced headaches with this pre-workout, which can sometimes interfere with their workouts. Still, this is a great supplement overall.

Get it Here.

2. Xwerks Ignite 


Xwerks Ignite Orange Pre Workout Powder includes herbs and nutrients that aid in dopamine production. It also contains Rhodiola, which encourages steady testosterone levels and a healthy sexual appetite.

The Rhodiola ingredient also helps stabilize physiological processes in the body. It makes your body more resilient to mental and physical stress. The pre-workout can boost energy, athletic performance, and cognition.

It’s naturally sweetened and has four flavors: blue raspberry, watermelon, orange, and green apple. 

Get it Here.

3. Bucked Up


Bucked Up Pre Workout was developed to take your training to the next level. The arginine and amino acids citrulline in this pre-workout promotes blood flow to counter muscle fatigue.

Betaine increases resistance and endurance training. It increases protein synthesis, leading to significant muscle gains and more strength: Taurine and beta-alanine boost muscle power. 

It comes in a caffeine-free option, although it still contains artificial sweeteners. A plus point is that it doesn’t have proprietary blends. So you can see exactly what ingredients and amounts go into your body. 

This pre-workout is best for bodybuilders, serious athletes, and anyone looking for energy, muscle growth, focus, and pump.

Get it Here.

Try a pre-workout and testosterone booster combo, such as 4 Gauge pre-workout and Prime Male. 

Post You May Like: Prime Labs Men’s Testosterone Booster Review

FAQs About Pre-Workout and Testosterone Boost

Can C4 pre-workout increase testosterone?

Yes, C4 Sport by Cellucor can increase testosterone. It contains testosterone-boosting ingredients, such as caffeine and micronized creatine monohydrate. However, its manufacturer does not market it for its testosterone-boosting benefits. Instead, it focuses on how it can optimize exercise to crush goals.

What exercise increases testosterone most?

Resistance exercises, such as push-ups, planks, deadlifts, and squats, can increase short and long-term testosterone levels. Strength, cardio, and endurance training may also boost testosterone levels. 

High-intensity interval training (HIIT) is also practical, although other workouts also boost T levels to some extent. [14] 

What gym supplements increase testosterone?

Opt for gym supplements that contain natural ingredients, such as D-aspartic acid, Panax ginseng, ashwagandha extract, green tea extract, vitamin D and zinc, and fenugreek extract.

Post You May Like: Gorilla Mode Pre-Workout Review


Does pre-workout boost testosterone?

(Source: Pexels)

Although there are pre-workouts that may boost testosterone levels, it is not a cure for testosterone deficiencies or people already experiencing low T symptoms. What it does instead is it decreases one’s chances of struggling with low testosterone.

So, check your testosterone levels first if your primary goal of taking the supplement is to boost your testosterone levels. You can use a testosterone test kit or talk to your healthcare provider for a testosterone level test for a more accurate result.

If you’re struggling and still need help finding the best pre-workout, please read our other reviews at
















Nathan Lloyd, MSc

I’m a personal trainer, based in Boulder, Colorado.
I service clients physically in the Boulder area, mainly in the ONE Boulder Fitness Gym, but am also available for online consulting and coaching.

If you’re interested in my personal coaching programs, please contact me via the contact page.